PCR is the abbreviation of Polymerase Chain Reaction. This method is a major innovation in gene amplification technology that can amplify a very small amount of target DNA millions of times. The iden...
PCR is the abbreviation of Polymerase Chain Reaction. This method is a major innovation in gene amplification technology that can amplify a very small amount of target DNA millions of times. The identification of the Coronavirus mainly relies on PCR nucleic acid detection technology. After the emergence of the Coronavirus, Chinese scientists have successfully discovered the specific nucleic acid sequence in the Coronavirus. If the specific nucleic acid sequence of the new coronavirus can be detected in the sample of a suspected patient, it is considered that the patient may be infected by the new coronavirus.
PCR technology is widely used in various clinical diagnosis due to its advantages such as strong specificity, high sensitivity, low requirements for test samples, and convenience. Currently, various clinical applications of PCR technology are being carried out in hospitals. The standardized design of PCR laboratories can ensure the reliability of test results. Therefore, the design and construction of PCR laboratories have received more and more attention from the government.
The key issue in PCR laboratory design is how to avoid contamination. In actual work, the following types of contamination are common: contamination of amplified products, contamination of natural genomic DNA, contamination of reagents, and contamination between samples. Therefore, to avoid pollution, the first priority should be prevention, not elimination.
The work area should be strictly divided: each experimental area must have obvious marks (such as warning signs or different ground colors, etc.) to avoid confusion of equipment, items, reagents, etc. in different experimental areas.
The PCR laboratory should be equipped with a reasonable system, a reasonable air-conditioning and ventilation system and strict airflow pressure control to ensure different pressure requirements in different experimental areas.
Standardized operation: The technicians of the clinical gene amplification testing laboratory must be trained on the job. During the experiment operation, the experimenter must wear gloves and change them frequently. The cleaning work should be timely and correct. After the experimental work is over, the area must be cleaned immediately.
Strict management: strictly control the personnel entering and leaving the laboratory. Use work clothes with distinctive signs (such as different colors) in each experimental area. When the staff leaves, they are not allowed to take the work clothes in this area to other areas. Reduce unnecessary walking in the experimental area to avoid cross-contamination. The amplification product analysis area is the most prone source of contamination, so the waste liquid cannot be dumped in the laboratory. It must be disinfected and discarded away from the laboratory. Disposable materials such as used tips should also be immersed in disinfectant for disinfection. After unified processing.
Guidelines for disinfection of PCR laboratory:
How to disinfect and decontaminate the entire space of the PCR laboratory, including air and objects. Dry fog hydrogen peroxide sterilizer can be used. FHP01CN with special hydrogen peroxide disinfectant can effectively kill microorganisms in the space and surface of the laboratory. And due to the low-concentration disinfectant, the risk of corrosion or aging of other equipment can be reduced.
1. Specifically for the source of nucleic acid pollution: the unique sterilization mechanism can ensure the effective elimination of mycoplasma. Because of its good diffusibility, it can ensure that the hidden sources of mycoplasma contamination will be eradicated.
2. Faster sterilization scheme for confined spaces: it can prevent environmental air pollution and aerosol pollution in PCR laboratory.
3. Non-toxic, no residue, safe and reliable, and provide customized disinfection solutions.
We are committed to the prevention and treatment of nucleic acid laboratory contamination. By introducing advanced technology and combining the actual conditions of the PCR laboratory, we have formulated a professional solution to remove nucleic acid contamination. And provide professional standard process operation and disinfection operation training.